Flow cytometry of human spermatozoa
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F. J. Otto; Ursula Hacker; J. Zante; J. Schumann; W. Göhde; M. L. Meistrich
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Article
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1979
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Springer
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English
⚖ 273 KB
Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochr