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Flow cytometry (FCM) of human epidermal cells

✍ Scribed by E. Thorud; G. Volden


Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
986 KB
Volume
269
Category
Article
ISSN
0340-3696

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Flow cytometry of human spermatozoa
✍ F. J. Otto; Ursula Hacker; J. Zante; J. Schumann; W. GΓΆhde; M. L. Meistrich πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 273 KB

Methods are given for the preparation and staining of human spermatozoa for flow cytometric DNA measurements. Using agents for the reductive cleavage of disulfide crosslinks and suitable proteolytic enzymes an effective decondensation of the sperm chromatin and a DNA-proportional uptake of fluorochr