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Flow Cytometry Identification of X- and Y-Chromosome-Bearing Goat Spermatozoa

✍ Scribed by I Parrilla; JM Vazquez; J Roca; EA Martinez

Publisher: John Wiley and Sons
Year: 2004
Tongue: English
Weight: 103 KB
Volume: 39
Category: Article
ISSN: 0936-6768
DOI: 10.1046/j.1439-0531.2003.00480.x

No coin nor oath required. For personal study only.

✦ Synopsis

Flow cytometric sorting technology was used to measure the difference in DNA content between X‐ and Y‐chromosome‐bearing spermatozoa in bucks. Spermatozoa were analysed by flow cytometry to characterize X‐ and Y‐chromosome‐bearing sperm populations and to quantify the DNA difference between them. Two symmetrical, overlapping and clearly separated peaks, corresponding to X‐ and Y‐bearing spermatozoa, were detected. The difference in fluorescence intensity between the peaks was 4.4 ± 0.03% without any significant inter‐ or intra‐animal variations. Therefore, the identification and selection of high‐purity samples of sperm populations for sex sorting is easier in bucks compared with other domestic species.

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Flow Cytometry Identification of X- and Y-Chromosome-Bearing Goat Spermatozoa

✦ Synopsis

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