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Fast conventional Fmoc solid-phase peptide synthesis with HCTU

✍ Scribed by Christina A. Hood; German Fuentes; Hirendra Patel; Karen Page; Mahendra Menakuru; Jae H. Park

Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
282 KB
Volume
14
Category
Article
ISSN
1075-2617

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✦ Synopsis

Abstract

1__H__‐Benzotriazolium 1‐[bis(dimethyl‐amino)methylene]‐5‐chloro‐hexafluorophosphate (1‐),3‐oxide (HCTU) is a nontoxic, nonirritating and noncorrosive coupling reagent. Seven biologically active peptides (GHRP‐6, ^65–74^ACP, oxytocin, G‐LHRH, C‐peptide, hAmylin~1–37~, and β‐amyloid~1–42~) were synthesized with reaction times reduced to deprotection times of 3 min or less and coupling times of 5 min or less using HCTU as the coupling reagent. Expensive coupling reagents or special techniques were not used. Total peptide synthesis times were dramatically reduced by as much as 42.5 h (1.8 days) without reducing the crude peptide purities. It was shown that HCTU can be used as an affordable, efficient coupling reagent for fast Fmoc solid‐phase peptide synthesis. Copyright © 2007 European Peptide Society and John Wiley & Sons, Ltd.

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