Facile synthesis of puromycin-tethered oligonucleotides at the 3′-end
✍ Scribed by Shuji Ikeda; Isao Saito; Hiroshi Sugiyama
- Book ID
- 108386934
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- French
- Weight
- 239 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Immobilization of an aliphatic amino-alcohol on a 2,2'-dithiodiethanol derivatized support via a carbamate linkage formation allows automated synthesis of oligonucleotides involving?%ino-alkyl derivatizations at their 3'-end.
A lipophilic phosphorylating agent was prepared and used for the synthesis of pentadeoxyribonucleotide with aminoethyl group at 5' end on a polymer support by the phosphotriester method. Chemically synthesized oligodeoxyribonucleotide with defined sequence is widely used as the primer for DNA sequen
The attachment of an unprotected aminoacid either at the 5' end of an oligonucleotide v&a a carbamate linkage or at the 3' end via a phosphoramidate bond leads to modified oligonucleotides containing carboxyl group via alinker. These carboxyl groupscan then specifically react with the primary amino