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Synthesis of oligonucleotides containing a carboxyl group at either their 5′ end or their 3′ end and their subsequent derivatization by an intercalating agent

✍ Scribed by Marina Gottikh; Ulysse Asseline; Nguyen T. Thuong


Publisher
Elsevier Science
Year
1990
Tongue
French
Weight
258 KB
Volume
31
Category
Article
ISSN
0040-4039

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✦ Synopsis


The attachment of an unprotected aminoacid either at the 5' end of an oligonucleotide v&a a carbamate linkage or at the 3' end via a phosphoramidate bond leads to modified oligonucleotides containing carboxyl group via alinker. These carboxyl groupscan then specifically react with the primary amino group ofxunomycin in the presence of carbodiimide.