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F.97. Effect of in vitro-expanded CD4+CD25+Foxp3+ Regulatory T Cell Therapy Combined with Lymphodepletion in Allotransplantation

โœ Scribed by Dong-Gyun Lim; Youn-Hee Park; Sun-Kyung Koo; Youngji Kim; Chan-Sik Park; Song-Cheol Kim; Duck-Jong Han

Book ID
113540352
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
41 KB
Volume
127
Category
Article
ISSN
1090-2341

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The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells
โœ Jacqueline P. Law; Dale F. Hirschkorn; Rachel E. Owen; Hope H. Biswas; Philip J. ๐Ÿ“‚ Article ๐Ÿ“… 2009 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 47 KB ๐Ÿ‘ 1 views

the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. F