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The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells

✍ Scribed by Jacqueline P. Law; Dale F. Hirschkorn; Rachel E. Owen; Hope H. Biswas; Philip J. Norris; Marion C. Lanteri

Publisher: John Wiley and Sons
Year: 2009
Tongue: English
Weight: 47 KB
Volume: 77A
Category: Article
ISSN: 0196-4763
DOI: 10.1002/cyto.a.20843

No coin nor oath required. For personal study only.

✦ Synopsis

the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. For the final wash of the BD-Foxp3 buffer, the authors used 2 ml of PBS instead of the 2 ml of permeabilization buffer described on page 3.

PBS was also used for all washes of the Caltag buffers.

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