the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. F
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells
β Scribed by Jacqueline P. Law; Dale F. Hirschkorn; Rachel E. Owen; Hope H. Biswas; Philip J. Norris; Marion C. Lanteri
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 568 KB
- Volume
- 75A
- Category
- Article
- ISSN
- 0196-4763
No coin nor oath required. For personal study only.
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## Abstract ## Background The purpose of this study was to investigate the presence of CD4^+^CD25^+^FOXP3^+^ regulatory T (Treg) cells both in peripheral blood and local tumors in patients with nasal inverted papilloma (NIP). ## Methods By using flow cytometry, the frequencies of CD4^+^CD25^+^FO
## Abstract Regulatory T cells (Treg cells) have been well documented to have a crucial physiological role in preventing the development of autoimmune diseases and keeping selfβtolerance. Foxp3, a recently identified member of the forkhead transcription factors, serves as a master regulator for the
The transcription factor FOXP3 plays a key role in CD4 + CD25 + regulatory T cell function and represents a specific marker for these cells. Despite its strong association with regulatory T cell function, in humans little is known about the frequency of CD4 + CD25 + cells that express FOXP3 protein