RNA extraction from mammalian tissue has been compared using the different deproteinizing agents: a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and
Extraction of RNA from mammalian tissue culture cells
โ Scribed by K.T. Atherton; G. Darby
- Book ID
- 107711543
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 453 KB
- Volume
- 57
- Category
- Article
- ISSN
- 0003-2697
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A rapid procedure for the isolation of total RNA from small amounts of mammalian tissue (35 to 150 mg) is described. Tissues were homogenized in the presence of RNase inhibitors but in the absence of strong detergents. Contaminants were removed by phenol/chloroform extraction and Sephadex column chr