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Efficient extraction of RNA from mammalian tissue

✍ Scribed by Marsha L. Frazier; Wendy Mars; Dagne L. Florine; Richard A. Montagna; Grady F. Saunders

Publisher: Springer
Year: 1983
Tongue: English
Weight: 728 KB
Volume: 56
Category: Article
ISSN: 0300-8177
DOI: 10.1007/bf00227211

No coin nor oath required. For personal study only.

✦ Synopsis

RNA extraction from mammalian tissue has been compared using the different deproteinizing agents: a) guanidine-HCl, b) guanidinium-thiocyanate, c) buffer-saturated phenol, or d) buffer-saturated phenol followed by a proteinase K digestion of the aqueous phase. Both solid tissues (first, second, and third trimester fetal bovine pancreas), and human white blood cell populations were studied. Degradation, as seen in citric acid-urea agarose gels, and the ability to serve as templates for cell-free protein synthesis were used as criteria to assess the efficiency of the different methods. We conclude that employing buffer-saturated phenol with proteinase K digestion is a superior method for consistent extraction of relatively undegraded RNA in quantitative amounts from mammalian tissue.

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