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Expression, purification, crystallization and preliminary X-ray diffraction analysis of nurse shark β2-microglobulin

✍ Scribed by Lu, Shuangshuang ;Yao, Shugang ;Chen, Rong ;Zhang, Nianzhi ;Chen, Jianmin ;Gao, Feng ;Xia, Chun


Book ID
114529366
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
485 KB
Volume
68
Category
Article
ISSN
1744-3091

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The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclin