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Expression, purification, crystallization and preliminary X-ray diffraction analysis of Deg5 from Arabidopsis thaliana

✍ Scribed by Fan, Haitian ;Sun, Wei ;Sun, Zhe ;Gao, Feng ;Gong, Weimin


Book ID
114529452
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
389 KB
Volume
68
Category
Article
ISSN
1744-3091

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The recombinant homodimeric hemoglobin from the strictly aerobe gram-negative bacterium Vitreoscilla stercoraria has been expressed in Escherichia coli, purified to homogeneity, and crystallized by vapor diffusion techniques, using ammonium sulfate as precipitant. The crystals belong to the monoclin