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Cloning, purification, crystallization and preliminary X-ray studies of human α1-microglobulin

✍ Scribed by Zhang, Yangli ;Gao, Zengqiang ;Zhang, Zhenzhen ;Luo, Miao ;Huang, Ailong ;Dong, Yuhui ;Wang, Deqiang


Book ID
114529418
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
318 KB
Volume
68
Category
Article
ISSN
1744-3091

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## Abstract Nonglycosylated α‐amylase, a major component of human parotid saliva, has been crystallized by the vapor diffusion technique using 2‐methyl‐2,4‐pentanediol as the precipitant in the presence of CaCl~2~ at pH 9.0. The crystals are orthorhombic, space group __P__2~1~2~1~2~1~ with unit cel