High level expression and simple purific
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Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-II Park; Se-Yong Lee; Young
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Article
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1995
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Springer Netherlands
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English
β 345 KB
A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of