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Expression and Purification of Recombinant Human Granulocyte Colony-Stimulating Factor in Fed-Batch Culture ofEscherichia coli

✍ Scribed by Kim, Chang-Kyu; Choi, Jun-Ha; Lee, Seung-Bae; Lee, Sang-Mahn; Oh, Jae-Wook


Book ID
121580686
Publisher
Springer-Verlag
Year
2014
Tongue
English
Weight
531 KB
Volume
172
Category
Article
ISSN
0273-2289

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A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of