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High-level secretory production of human granulocyte-colony stimulating factor by fed-batch culture of recombinantEscherichia coli

✍ Scribed by S. Yim; K. Jeong; H. Chang; S. Lee


Publisher
Springer
Year
2001
Tongue
English
Weight
151 KB
Volume
24
Category
Article
ISSN
1615-7605

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A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of