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Expression, purification, and in vitro biological activities of recombinant bovine granulocyte-colony stimulating factor

โœ Scribed by Mohammad Heidari; James A. Harp; Marcus E. Kehrli Jr.

Book ID
117335799
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
201 KB
Volume
81
Category
Article
ISSN
0165-2427

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A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of