A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of
โฆ LIBER โฆ
Renaturation and Purification of Biologically Active Recombinant Human Macrophage Colony-Stimulating Factor Expressed in E. Coli
โ Scribed by Halenbeck, Robert ;Kawasaki, Ernest ;Wrin, Joe ;Koths, Kirston
- Book ID
- 109905552
- Publisher
- Nature Publishing Group
- Year
- 1989
- Tongue
- English
- Weight
- 741 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1087-0156
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## Abstract Stem cell factor (SCF) is a multifunctional cytokine involved in hematopoiesis, melanogenesis and gametogenesis. Previous studies have demonstrated that avian SCF is a requirement for the proliferation and survival of various cell types __in vivo__ and __in vitro__. In the current study