Expansion of circulating Foxp3+CD25bright CD4+ T cells during specific venom immunotherapy
β Scribed by M. C. Pereira-Santos; A. P. Baptista; A. Melo; R. R. Alves; R. S. Soares; E. Pedro; M. Pereira-Barbosa; R. M. M. Victorino; A. E. Sousa
- Book ID
- 108688147
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 142 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0954-7894
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## Abstract ## Background The purpose of this study was to investigate the presence of CD4^+^CD25^+^FOXP3^+^ regulatory T (Treg) cells both in peripheral blood and local tumors in patients with nasal inverted papilloma (NIP). ## Methods By using flow cytometry, the frequencies of CD4^+^CD25^+^FO
the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. F