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Exchange of the valine 2-H in the biosynthesis of L-δ-(α-aminoadipoyl)-L-Cysteinyl-D-valine

✍ Scribed by Jack E. Baldwin; Michael F. Byford; Robert A. Field; Chia-Yang Shiau; Wendy J. Sobey; Christopher J. Schofield

Publisher
Elsevier Science
Year
1993
Tongue
French
Weight
423 KB
Volume
49
Category
Article
ISSN
0040-4020

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✦ Synopsis

Incubanon of [2-2H]-valme with punfled ACV synthetase from both Cephalosporlum acremontum and Streptomyces clavulrgerus produced L-&(a-ammoadqoyl)-L-cystemyl-D-vahne (ACV), determmed by the essentzilly complete (>95%) loss of deutenum from the a posmon of the Incorporated vahne Incubations with deutermm oxide/water as solvent produced ACV Hnth slgmficant mcorporatton of deutermm mto the valmyl residue These observations confirm the prior proposal that a single mulafuncttonal enzyme IS responsible for both the formatton of the peptlde bonds of ACV and the eplmensanon of the valmyl residue

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