Evaluation of three procedures for the estimation of serum digoxin levels

✍ Scribed by M. Givner; R. Gaudet; S. Wakkary; D. Ryder; S. Boss; M. Moss; C. Jacklyn

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 104 KB
• Volume: 26
• Category: Article
• ISSN: 0009-9120
• DOI: 10.1016/0009-9120(93)90050-g

✦ Synopsis

Prior to GC-MS analysis, samples (1 mL) were passed through a C-18 SPE cartridge (Supelco) preconditioned with ethyl acetate, 96% (v/v) ethanol and 10% (v/v) ethanol in sequence. Resveratrol was eluted with ethyl acetate and 1 mL was collected of which 1 I~L was injected. For samples with very low concentration, the eluate was dried and reconstituted in 200 I~L ethyl acetate. The method gave the following performance characteristics: sensitivity 10 pg on injection or 10 p.p.b, in sample; CV within-batch 1.7%-5.7%; CV day-to-day <8%; quantitative recovery (-100%); linearity 10-40 p.p.b, for human urine and 60-300 p.p.b, for human serum. The method should have wide application in clinical, pharmacological and industrial investigations and is the first step in a program to develop formulations containing resveratrol (food, wine or pharmaceuticals) for human consumption.