Evaluation of phytic acid as a buffer additive for the separation of proteins in capillary electrophoresis
โ Scribed by J.R. Veraart; Y. Schouten; C. Gooijer; H. Lingeman
- Book ID
- 108340957
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 519 KB
- Volume
- 768
- Category
- Article
- ISSN
- 1873-3778
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## Abstract In CZE, the adsorption of the proteins on the capillary wall is a common problem. This paper describes the simple method of utilizing zwitterionic buffer additives to improve the separation of proteins in untreated fused silica capillaries at neutral pH. Three kinds of zwitterion are ev
Studies are reported on the effect of the sodium salt of phytic acid on the resolution of peptides and proteins. Improved separation in the case of peptides is shown to be due to ion-ion pairing interactions between the positively charged peptides and the phytic acid polyanionic species. The improve