Separation of acidic and basic proteins by capillary electrophoresis using gemini surfactants and gemini-capped nanoparticles as buffer additives
β Scribed by Qian Liu; YanQing Li; YanMin Yang; ShouZhuo Yao
- Book ID
- 107348925
- Publisher
- SP Science China Press
- Year
- 2009
- Tongue
- English
- Weight
- 734 KB
- Volume
- 52
- Category
- Article
- ISSN
- 1674-7291
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π SIMILAR VOLUMES
Incorporation of a low concentration of cetyltrimethylammonium bromide (CTAB) in the running electrolyte is shown to dynamically coat the silica capillary and to reverse the direction of electroosmotic flow. The CTAB coating prevented interaction of proteins with the capillary surface and enabled sh
## Abstract This report describes a method for enrichment and separation of acidic and basic proteins using the centrifugal ultrafiltration followed by nanoparticleβfilled capillary electrophoresis. To improve stacking and separation efficiencies of proteins, the separation buffer containing 1.6% p