Separation of basic proteins by capillary electrophoresis using cross-linked polyacrylamide-coated capillaries and cationic buffer additives
β Scribed by A. Cifuentes; M. de Frutos; J.M. Santos; J.C. Diez-Masa
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 890 KB
- Volume
- 655
- Category
- Article
- ISSN
- 1873-3778
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Incorporation of a low concentration of cetyltrimethylammonium bromide (CTAB) in the running electrolyte is shown to dynamically coat the silica capillary and to reverse the direction of electroosmotic flow. The CTAB coating prevented interaction of proteins with the capillary surface and enabled sh
## Abstract This report describes a method for enrichment and separation of acidic and basic proteins using the centrifugal ultrafiltration followed by nanoparticleβfilled capillary electrophoresis. To improve stacking and separation efficiencies of proteins, the separation buffer containing 1.6% p