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The Effect of Phytic Acid on the Resolution of Peptides and Proteins in Capillary Electrophoresis

โœ Scribed by G.N. Okafo; H.C. Birrell; M. Greenaway; M. Haran; P. Camilleri

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
351 KB
Volume
219
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Studies are reported on the effect of the sodium salt of phytic acid on the resolution of peptides and proteins. Improved separation in the case of peptides is shown to be due to ion-ion pairing interactions between the positively charged peptides and the phytic acid polyanionic species. The improved peak shapes related to the proteins can be interpreted in terms of the sample preconcentration due to injection of analytes from a water medium to one of high ionic strength.

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โœ Nong Chen; Lei Wang; Yukui Zhang ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 459 KB ๐Ÿ‘ 1 views

It was found that unlike the electroosmotic flow, the electrophoretic mobilities of small peptides in capillary zone electrophoresis (CZE) were invariant over the buffer concentration range studied. Migration of these peptides in CZE is a electroosmotic-governed process. The quantitative linear rela