Effects of buffer concentration on the electrophoretic behaviors of small peptides in capillary zone electrophoresis

✍ Scribed by Nong Chen; Lei Wang; Yukui Zhang

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 459 KB
• Volume: 7
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/mcs.1220070302

✦ Synopsis

It was found that unlike the electroosmotic flow, the electrophoretic mobilities of small peptides in capillary zone electrophoresis (CZE) were invariant over the buffer concentration range studied. Migration of these peptides in CZE is a electroosmotic-governed process. The quantitative linear relationships between the logarithm of migration times (log t m ) and the reciprocal of column temperature were investigated under different buffer concentrations to study the activation energies of diffusion (AED) as the function of the buffer concentration. The preexponential factors were quantitatively correlated with the structural parameters of the peptides studied. o 1995 John Wiley & Sons, Inc.