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Evaluation of high performance liquid chromatography/electrospray mass spectrometry with selected ion monitoring for the analysis of large synthetic combinatorial peptide libraries

✍ Scribed by Pierre-Hervé Lambert; Jean A. Boutin; Sophie Bertin; Jean-Luc Fauchère; Jean-Paul Volland

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
132 KB
Volume
11
Category
Article
ISSN
0951-4198

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✦ Synopsis

Liquid chromatography/electrospray mass spectrometry with selected-ion monitoring makes possible the visualization and positive identification of a family of isobaric ions from libraries comprising hundreds of peptides. For larger libraries (up to 10 5 peptides), a given peptide, known to be unique at a given m/z, was detected, but the full characterization of others at other m/z values from the library is mainly limited by LC separation efficiency. However, this approach offers new possibilities to evaluate the completeness of combinatorial libraries.

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