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Evaluation of enzyme-linked immunosorbent assay and liquid chromatography–tandem mass spectrometry methodology for the analysis of 8-oxo-7,8-dihydro-2′-deoxyguanosine in saliva and urine

✍ Scribed by Marcus S. Cooke; Rajinder Singh; Georgina K. Hall; Vilas Mistry; Tiago L. Duarte; Peter B. Farmer; Mark D. Evans

Book ID: 113630113
Publisher: Elsevier Science
Year: 2006
Tongue: English
Weight: 269 KB
Volume: 41
Category: Article
ISSN: 0891-5849
DOI: 10.1016/j.freeradbiomed.2006.09.009

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The renally excreted amount of 8-oxo-7,8-dihydro-2'-deoxyguanosine (oxo(8)dG) is a potential marker of oxidative DNA damage by reactive oxygen species. We have developed a high-performance liquid chromatographic (HPLC) method to determine oxo(8)dG in urine from humans and Wistar rats. First, 300 mic