A simple and cost effective method for the quantification of 8-hydroxy-2′-deoxyguanosine from urine using liquid chromatography tandem mass spectrometry

✍ Scribed by Brian Crow; Michael Bishop; Kasey Kovalcik; Dean Norton; Joe George; J. Alexander Bralley

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 281 KB
• Volume: 22
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.946

✦ Synopsis

Abstract

A rapid and high‐throughput method for the determination of urinary levels of the oxidative stress biomarker, 8‐hydroxy‐2′‐deoxyguanosine (8‐OH‐dG), has been developed and validated using liquid chromatography combined with electrospray ionization tandem mass spectrometry (LC‐MS/MS). The assay features a cheap and readily available non‐isotopic internal standard, a single‐step filtration sample preparation, and a total analysis time of 6 min including column re‐equilibration. The method was validated based on linearity, accuracy (100–106%), precision (CV < 7%), sample preparation stability (≤5%, 72 h). Intra‐laboratory patient ranges were established comparing children and adults (n = 345). Copyright © 2007 John Wiley & Sons, Ltd.