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Evaluation of a sensitive detection method for peptide arrays prepared by SPOT synthesis

✍ Scribed by A. Dürauer; E. Kopecky; E. Berger; M. Seifert; R. Hahn; A. Jungbauer

Book ID
113681751
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
378 KB
Volume
66
Category
Article
ISSN
0165-022X

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Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H,A, H,B, and H4) prepared from oocytes ofxenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion ofoocy