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A rapid and sensitive method for the detection of histone peptides

โœ Scribed by Dennis J. O'Kane; Robert G. Levenson; Raymond F. Jones

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
977 KB
Volume
86
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H,A, H,B, and H4) prepared from oocytes ofxenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion ofoocyte histones of X. laevis and the peptic digestion of the /3 chain of insulin. Using the /3 chain of insulin for a comparison, the 8 major peptide fragments could be separated by electrophoresis within 30 min and were detectable at the picomole level. Some 70 peptide spots of X. laevis oocyte histones were resolved, thus permitting the analysis of this complex mixture of polypeptides without the need for prior separation.

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