A new technique for the detection of amylase isoenzymes is described. An amylase sensitive test paper is applied to the surface of a flat bed electrophoresis gel and the isoenzyme bands rapidly appear as well defined white lines on a pink background. The electrophoresis gel is undamaged by this trea
A sensitive, rapid and specific technique for the detection of collagenase using zymography
β Scribed by Kawl Thanzami; Ipsita Roy
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 253 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
β¦ Synopsis
Abstract
A fast and specific method for the detection of collagenase by electrophoresis is described. The method avoids inclusion of the substrate in the resolving gel and can detect nanogram levels of the enzyme.
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