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A rapid chromatographic technique for the detection of dye-binding proteins

✍ Scribed by Alan G. Clark; Sie Ting Wong

Publisher: Elsevier Science
Year: 1978
Tongue: English
Weight: 396 KB
Volume: 89
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(78)90357-3

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✦ Synopsis

A rapid and inexpensive assay for dye-binding proteins has been developed. It depends on the separation of free and protein-bound sulfobromophthalein in I-ml columns of Sephadex G-25 due to differential adsorption of the dye to the protein and to the Sephadex. With bovine serum albumin the calibration curve is linear between 0.03 and 3 mg of protein and is not affected by the presence of moderate concentrations of salt.

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