A new method has been developed for the rapid determination of D-cysteine contents in synthetic peptides. It is based on the reduction of cystine residues, when present, with tris-alkylphosphines, selective derivatization of the cysteine residues with 4-vinylpyridine, followed by acid hydrolysis of
A sensitive method for the detection of racemization in peptide synthesis
✍ Scribed by E. Taschner; Ł. Lubiewska; M. Smulkowski; H. Wojciechowska
- Book ID
- 112700283
- Publisher
- Springer
- Year
- 1968
- Tongue
- English
- Weight
- 519 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1420-682X
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## Abstract A suitably protected dipeptide of configuration L‐D, __e.g.__ Z‐L‐Ala‐D‐Ala is coupled with an all L alanine peptide, __e.g.__ L‐Ala‐L‐Ala‐ONbAbbreviations according to the IUPAC‐IUB rules, ‘Symbols for Amino‐Acid Derivatives and Peptides, Recommendations (1971)’'. see __e.g.__ J. biol.
Fluorescamine has been used to obtain a peptide map of a mixture of histones (H3, H,A, H,B, and H4) prepared from oocytes ofxenopus laevis. Fluorescamine was found to be more sensitive than o-phthalaldehyde or ninhydrin-Cd for the detection of peptide fragments obtained from tryptic digestion ofoocy