We describe a sensitive new method for specific detection and quantitation of phospholipid-binding proteins using two purified lipocortins as model proteins. The method consists of blotting proteins to nitrocellulose membranes followed by incubation with radiolabeled phospholipids and autoradiograph
โฆ LIBER โฆ
Method for homogeneous spotting of antibodies on membranes: application to the sensitive detection of ochratoxin A
โ Scribed by Debjani Saha; Debopam Acharya; Tarun K. Dhar
- Publisher
- Springer
- Year
- 2006
- Tongue
- English
- Weight
- 218 KB
- Volume
- 385
- Category
- Article
- ISSN
- 1618-2650
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## Abstract Three methods for the conjugation of oligonucleotides to antibodies and the subsequent application of these conjugates to protein detection at attomole levels in immunoassays are described. The methods are based on chemical modification of both antibody and oligonucleotide. Aldehydes we
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