Evaluation of a gas chromatographic method for the quantitative estimation of hexoses from neutral glycolipids

✍ Scribed by R. Kannan; P.N. Seng; H. Debuch

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 665 KB
• Volume: 92
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)97193-0

✦ Synopsis

The alditol acetate method for the estimation of the hexoses from the carbohydrate moiety in neutral glycolipids has been standardized using gas-liquid chromatographic conditions which provided better resolution of the individual neutral sugars. The method involves the aqueous acid hydrolysis of the glycolipid, reduction of the released free sugars to the alcohols and acetylation before gas chromatographic analysis. Glucose-containing lipids differ from galactolipids in that the former require a longer time of hydrolysis for a quantitative cleavage of the sugar from the ceramide moiety. Results of the application of this method to glycolipids in the range of 0.2 to 0.5 ,umole gave quantitative values using arabinose as internal standard. The sugar estimations of cerebrosides and sulphatides in the presence of phospliatides also gave quantitative results. The method has also been applied to determine the galactose to glucose ratio of ceramide di-, tri-and tetrahexosides of human erythrocyte ghost lipids.