𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A validated gas chromatographic method for the evaluation of enzymatic enantioselectivity in kinetic resolution applications

✍ Scribed by Ykelien L. Boersma; Paula C. Scheltinga; Melloney J. Dröge; Rein Bos; Wim J. Quax

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
73 KB
Volume
28
Category
Article
ISSN
1615-9306

No coin nor oath required. For personal study only.

✦ Synopsis

An enantioselective gas chromatography (GC) method has been developed and validated for determination of the enantiomers of citronellol in kinetic resolution experiments. S-(-)-beta-Citronellol is a precursor of rose oxide. After solid-phase extraction (SPE) with ethyl acetate, the enantiomers of R-(+)-beta-citronellol and S-(-)-beta-citronellol and their corresponding acetate- and butyrate esters were separated through enantioselective GC respectively. The method was validated and found to be reproducible, specific, accurate, and precise. Analyte recoveries and detection limits were also determined. The applicability of this method was shown in a kinetic resolution experiment using lipase A of Bacillus subtilis.

📜 SIMILAR VOLUMES

Development and validation of a gas chro
Development and validation of a gas chromatography-mass spectrometry method for the determination of phenazopyridine in rat plasma: application to the pharmacokinetic study
✍ Qinhua Chen; Kaijun Li; Zhuo Zhang; Peng Li; Jia Liu; Qiang Li 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 123 KB 👁 2 views

## Abstract Phenazopyridine hydrochloride is a strong analgesic used in the treatment of urinary tract infections. The aim of the present study was to develop a procedure based on gas chromatography‐mass spectrometry (GC‐MS) for the analysis of phenazopyridine in rat plasma. The method was set up a