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Ethanol improves the transformation efficiency of intact yeast cells

✍ Scribed by Vit Lauermann

Book ID
104717355
Publisher
Springer-Verlag
Year
1991
Tongue
English
Weight
284 KB
Volume
20
Category
Article
ISSN
0172-8083

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✦ Synopsis

A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies with different yeast strains and plasmids and up to a maximum of a 15-fold increase was observed.

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High efficiency transformation of intact
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
✍ Robert H. Schiestl; R. Daniel Gietz πŸ“‚ Article πŸ“… 1989 πŸ› Springer-Verlag 🌐 English βš– 780 KB

A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 X 105 transformants per microgram of vector DNA and to 1.5 % transformants per viable cell. The use of single stranded, or he