Improved method for high efficiency transformation of intact yeast cells
β Scribed by Gietz, Daniel (author);Jean, Andrew St (author);Woods, Robin A. (author);Schiestl, Robert H. (author)
- Book ID
- 111865141
- Publisher
- Oxford University Press
- Year
- 1992
- Tongue
- English
- Weight
- 106 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0305-1048
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A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies
A method, using LiAc to yield competent cells, is described that increased the efficiency of genetic transformation of intact cells of Saccharomyces cerevisiae to more than 1 X 105 transformants per microgram of vector DNA and to 1.5 % transformants per viable cell. The use of single stranded, or he