Pretreatment of yeast cells with lithium acetate (LiAc) and dithiothreitol (DTT) enhances the frequency of transformation by electroporation. The method shows improvements of 6-67-fold in wild-type strains derived from commonly used Saccharomyces cerevisiae genetic backgrounds. In addition, 15-300-f
โฆ LIBER โฆ
Cryopreservation of competent intact yeast cells for efficient electroporation
โ Scribed by Minoru Suga; Masaharu Isobe; Toyomasa Hatakeyama
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 117 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0749-503X
No coin nor oath required. For personal study only.
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A polymerase chain reaction (PCR)-based technique is described which allows for the determination of library plasmid insert DNA sequence directly and rapidly from intact yeast cells. Yeast spheroplasts are used to template a PCR reaction to amplify the insert sequence. This PCR product is then purif