Dithiothreitol Improves the Efficiency of Yeast Transformation
โ Scribed by A. Reddy; F. Maley
- Book ID
- 102560414
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 174 KB
- Volume
- 208
- Category
- Article
- ISSN
- 0003-2697
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A technique is described in which ethanol is used to improve the genetic transformation of intact yeast (Saccharomyces cerevisiae) cells pretreated with LiAc and PEG. Transformation efficiency was increased with increasing concentrations of ethanol with a peak at 10% concentration. The effect varies
Transformation of filamentous fungi with DNA has been hampered by the low frequencies generally obtained. A variety of methods have been explored, most relying on the enzymatic removal of the cell wall (protoplasting) before DNA treatment (reviewed in (1)). Dithiothreitol (DTT) 4 has been shown to i