Commercially available RNA and enzyme preparations are monitored for DNA contamination. The method used involves reaction with p-nitrophenylhydrazine and separation of the reaction products by thin-layer chromatography. When this technique is used, RNA preparations and bovine pancreas ribonuclease a
Estimation of DNA, RNA and 125I- and 3H-labelled DNA in the same sample
β Scribed by Roger F. Martin; George S. Hodgson
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 454 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In the DNA and RNA assays carried out with the use of ethidium bromide in cell and tissue homogenates according to the method of Karsten and Wollenberger (1). Pronase can be replaced to advantage by heparin in the nucleoprotein dissociation step. Furthermore, rhodamine B is used as a standard instea
## Abstract Analysis of the formation and repair of structurally modified DNA is of particular interest in the study of carcinogenesis, cancer therapy and aging. The quantification of specific DNA lesions by sensitive immunoanalytical methods requires radiotracers with high specific activity. We de
A simple, rapid, and sensitive method for the measurement of DNA, RNA, and protein synthesis in cell samples is presented. Tke method measures the amount of isotope incorporated into these macromolecules after cell collection on filter paper and washing with a methanol:chloroform:water mixture. Samp
A method is described for the differential estimation of DNA and RNA from rat liver on filter paper disks. To differentiate between DNA and RNA, samples are subjected to alkaline hydrolysis in microtitration plates prior to absorption on the disks. This procedure eliminates the loss of DNA which is