✦ LIBER ✦

An adaptation of the filter paper disk technique for the differential estimation of DNA and RNA of rat liver

✍ Scribed by Anthony E. Sobota

Publisher: Elsevier Science
Year: 1976
Tongue: English
Weight: 357 KB
Volume: 75
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(76)90103-2

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✦ Synopsis

A method is described for the differential estimation of DNA and RNA from rat liver on filter paper disks. To differentiate between DNA and RNA, samples are subjected to alkaline hydrolysis in microtitration plates prior to absorption on the disks. This procedure eliminates the loss of DNA which is observed if the disks are subjected to RNA hydrolysis after the sample has been absorbed on disks. The procedure is directly comparable to standard methods for the measurement of nucleic acids. 478

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