## Saccharopine (c-N-n-glutaryl-2) -n-lysine is a precursor of lysine biosynthesis in yeast and other fungi through the a-aminoadipic acid pathway (1). In Saccharomyces, saccharopine is converted into lysine in the presence of NAD\* at pH 10 or above. The reaction is catalyzed by the enzyme known
Enzymic synthesis of uridine diphosphoglucose-14C
โ Scribed by D.K. Fitzgerald; K.E. Ebner
- Publisher
- Elsevier Science
- Year
- 1966
- Tongue
- English
- Weight
- 203 KB
- Volume
- 15
- Category
- Article
- ISSN
- 0003-2697
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## Abstract Crude cellโfree extracts of Escherichia coli AB 259 Hfr 3000, thiโperform multistep conversion of ^14^Cโuracil to ^14^CโUTP in vitro in the presence of Dโribose/or riboseโ5โphosphate/, ATP and ATPโregenerating system. ATP concentration is crucial for significant ^14^CโUTP production. Qu
A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethylen
Uridine diphosphoglucose pyrophosphorylase was purified 2500-fold from rabbit skeletal muscle with a total recovery of 35% of the initial activity. The present procedure was made possible by an extensive use of hydrophobic chromatography. Purified pyrophosphorylase had a specific activity of 500 mum