Analysis of glucose 1-phosphate at the picomole level with [14C]UTP and uridine 5′-diphosphoglucose pyrophosphorylase
✍ Scribed by Chi P. Cheung; Robert J. Suhadolnik
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 200 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethyleneimine cellulose or by separating both labeled compounds on one-dimensional polyethyleneimine thin-layer chromatograms. The sensitivity of the method for glucose l-phosphate analysis is 5 pmol. The method has been successfully employed to monitor the level of glucose l-phosphate in early germination of wheat embryos.