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Enzymic assay of adenosine 3′,5′-monophosphate

✍ Scribed by N.S. Scott; I.R. Falconer

Publisher
Elsevier Science
Year
1965
Tongue
English
Weight
178 KB
Volume
13
Category
Article
ISSN
0003-2697

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✦ Synopsis

Adenyl cyclase and 3',5'-AMP1 have been implicated in the hormonal control mechanism of several biochemical reactions (1) and therefore the measurement of 3',5'-AMP in biological material is of interest. In the method of assay used by Rall and Sutherland (2, 3) and later modified by Brown, Clarke, Roux, and Sherman (4), a dog liver extract was used as the source of the relatively inactive liver dephosphophosphorylase and its kinase and of the active liver phosphorylase and its phosphatase. This preparation was shown to carry out the following reaction:

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