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Simultaneous isolation of adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (cGMP) in small tissue samples

✍ Scribed by C.C. Mao; A. Guidotti

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
348 KB
Volume
59
Category
Article
ISSN
0003-2697

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✦ Synopsis

A column chromatogrnphic sy;itrm has been developed for isolation and purification of cAMP and cGMP simultnneously from small tissue samples. In this system, a column of neutral aluminum oxide equilibrated in Tris-HCl buffer, pH 7.5 was used to purify cyclic nucleotides from olhe~ nucleotides in non-cyclic form, Subsequently, CAMP and cGMP are scparakd from each other through a Dowex l-52 column cluted with small volumes of HCl of d&rent molaritics. The elution patterns of both cyclic nuclcotides through the entire purification procedure arc highly reproducible with excellent recovery.

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