A coupled recording assay of cyclic AMP phosphodiesterase activity at and below 1 PM is presented. The method is especially useful in preparative work where speed is more important than high accuracy, but results agree well with a more accurate radioactive assay. Attention is drawn to the fact that,
A Semiautomated Method for the Assay of Cyclic Adenosine 5′-Monophosphate Phosphodiesterase
✍ Scribed by Donald V. Daniels; Robert Alvarez
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 81 KB
- Volume
- 236
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
A procedure for the assay of cyclic nucleotide phosphodiesterase is described in which labeled cyclic nucleotide is separated from labeled nucleoside by the batchwise addition of ethanolic slurries of Dowex 2 fluoride. Under the conditions described there is no detectable adsorption of nucleoside by
ferase reporter gene construct under the control of a PCK In cultured rat hepatocytes, the gluconeogenic key enzyme, phosphoenolpyruvate carboxykinase (PCK), is induced by gene promoter fragment (base 0979 to base /32). Luciferase activity was determined after stimulation of the cells with glucagon
## Abstract ## BACKGROUND The 32‐kilodalton dopamine and cyclic adenosine 3′,5′‐monophosphate‐regulated phosphoprotein (DARPP‐32) is the only known protein that can act as a protein phosphatase‐1 inhibitor or a protein kinase inhibitor. It is known as a neurogenic protein that plays a major role i
Elevated thyroid-stimulating hormone (TSH) and hypercholesterolemia commonly coexist, as typically seen in hypothyroidism, but there is no known mechanism directly linking the two. Here, we demonstrated that in liver cells, TSH promoted the expression of 3-hydroxy-3-methyl-glutaryl coenzyme A reduct