✦ LIBER ✦

Rapid recording luminometric assay of adenosine 3′,5′-cyclic monophosphate phosphodiesterase activity and a possible pitfall in coupled enzyme assays

✍ Scribed by Kari Suoranta; John Londesborough

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 308 KB
Volume: 126
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90119-1

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✦ Synopsis

A coupled recording assay of cyclic AMP phosphodiesterase activity at and below 1 PM is presented. The method is especially useful in preparative work where speed is more important than high accuracy, but results agree well with a more accurate radioactive assay. Attention is drawn to the fact that, because the product of the coupled reaction, ATP, is also an intermediate, its instantaneous rate of formation can exceed the rate of the first step under certain conditions.