Enzyme thermistor determination of glucose in serum using immobilized glucose oxidase
β Scribed by B. Danielsson; Kerstin Gadd; B. Mattiasson; K. Mosbach
- Book ID
- 115821493
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 842 KB
- Volume
- 81
- Category
- Article
- ISSN
- 0009-8981
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A flow-injection system with anodic aniperometric detection for glucose determination in human blood serum samples was optimized by a comparison of various arrangements of enzyme immobilization and the elimination o f interferences. The best results were obtained using a compact membrane biosensor i
During the continuing rapid daelopment of analytical methods based on immobilized enzymes, several techniques have been described for the determination of glucose. For example, glucose oxidase (E.C.1.1.3.4) entrapped in polyacrylamide gel was used by Updike and Hicks in two methods. In one case [l]
A flow-mqectlon system for the chemdummometnc determmatlon of o-glucose m serum with an lmmobdlzed pyranose oxldase reactor IS described Pyranose oxldase IS lmmobdlzed on controlled-pore glass beads and packed mto a stamless-steel column Serum (100 nl) IS dduted lOWfold with water Sample solution (1